Characterization of the asialoglycoprotein receptor on isolated rat hepatocytes.
نویسندگان
چکیده
منابع مشابه
Recycling of the Hepatic Asialoglycoprotein Receptor in Isolated Rat Hepatocytes
We recently reported that the dissociation of internalized receptor-1261-asialo-orosomucoid (ASOR) complexes by isolated hepatocytes is a biphasic process; most complexes dissociate rapidly but 25-50% dissociate slowly (Oka, J. A., and Weigel, P. H. J. Biol. Chern. 258, 10253-10262). Cells were allowed to endocytose a pulse of surface-bound '''I-ASOR, and were washed and then incubated at 37 "C...
متن کاملCharacterization of the Asialoglycoprotein Hepatocytes*
Rat hepatocytes, freshly isolated by a collagenase perfusion technique, bound [3H]asialo-orosomucoid in a sugar-specific and calcium-dependent manner as expected for the hepatic asialoglycoprotein receptor. At least 90% of the total cell surface-bound [3H]asialo-orosomucoid represented specific binding and could be removed by washing with EDTA. Freshly isolated cells had about 7 X lo4 surface r...
متن کاملHyperphosphorylation of the asialoglycoprotein receptor in isolated rat hepatocytes following ethanol administration.
Ethanol administration leads to altered function and impaired receptor-mediated endocytosis of the hepatocyte asialoglycoprotein receptor (ASGP-R). The purpose of the present study was to examine the effects of ethanol on the phosphorylation of the ASGP-R to determine whether this post-translational modification could contribute mechanistically to the observed ethanol-induced alterations. The m...
متن کاملLipoprotein secretion by isolated rat hepatocytes: characterization
Lipoprotein production by freshly isolated rat hepatocytes in suspension was studied during short (1 -3 hr) incubation periods. The hepatocytes release very low density (d < 1.01 g/ml) lipoprotein (VLDL) particles, which as a group a) contain triacylglycerols, phospholipids, free cholesterol, and cholesteryl esters in molar proportions of 100:21:8:4; b) have a mean diameter 1.5-fold larger than...
متن کاملExpression of the asialoglycoprotein receptor in cultured rat hepatocytes is modulated by cell density.
The influence of cell density on expression of the asialoglycoprotein receptor system in primary cultures of rat hepatocytes was evaluated by measuring the level of the receptor specific mRNA. When the hepatocytes are cultured at high cellular density and are not in a proliferative condition, the transcript molecules of the receptor appear increased about 50% with respect to the low plating den...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1980
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)43708-8